Native structure of a type IV secretion system core complex essential for Legionella pathogenesis.
Clicks: 178
ID: 100105
2014
Bacterial type IV secretion systems are evolutionarily related to conjugation systems and play a pivotal role in infection by delivering numerous virulence factors into host cells. Using transmission electron microscopy, we report the native molecular structure of the core complex of the Dot/Icm type IV secretion system encoded by Legionella pneumophila, an intracellular human pathogen. The biochemically isolated core complex, composed of at least five proteins--DotC, DotD, DotF, DotG, and DotH--has a ring-shaped structure. Intriguingly, morphologically distinct premature complexes are formed in the absence of DotG or DotF. Our data suggest that DotG forms a central channel spanning inner and outer membranes. DotF, a component dispensable for type IV secretion, plays a role in efficient embedment of DotG into the functional core complex. These results highlight a common scheme for the biogenesis of transport machinery.
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Authors | Kubori, Tomoko;Koike, Masafumi;Bui, Xuan Thanh;Higaki, Saori;Aizawa, Shin-Ichi;Nagai, Hiroki; |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Year | 2014 |
DOI | 10.1073/pnas.1404506111 |
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