defined nano-structuring with ultrashort pulses in gelatin biopolymer films for tissue-engineering

We compare the morphological modifications in gelatin films, induced with an 800nm femtosecond amplifier, in subject of the number of input pulses and the input pulse duration. Foam is formed in all cases, but only under certain conditions the desirable structures that mimic the native subcellular matrix are formed. The number of input pulses significantly changes the foam structure, allowing a well-defined nano-structuring for optimal tissue-engineering.