a comparative identification of ochratoxin a in longan fruit pulp by high performance liquid chromatography-fluorescence detection and electron spray ionization-mass spectrometry
Clicks: 203
ID: 182665
2010
Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
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| Authors | ;Jing Li;Haihui Xie;Bao Yang;Xinhong Dong;Linyan Feng;Feng Chen;Yueming Jiang |
| Journal | Journal of ethnopharmacology |
| Year | 2010 |
| DOI | 10.3390/molecules15020680 |
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