A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs.
Clicks: 241
ID: 20673
2019
A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values against the initial concentration of pathogen in the sheep sample.
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| Authors | Macori, Guerrino;McCarthy, Siobhán C;Burgess, Catherine M;Fanning, Séamus;Duffy, Geraldine; |
| Journal | Journal of microbiological methods |
| Year | 2019 |
| DOI | S0167-7012(19)30567-6 |
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| Keywords | Keywords not found |
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