Ovarian cancer. Protocol for the preparation of banded metaphases from tumor tissue.
Clicks: 29
ID: 277920
1992
Protocols are presented for preparation of banded metaphases for cytogenetic analysis from short-term and long-term cultures of ovarian cancer. For short-term cultures (less than 24 hours of culture of freshly dissociated tumor material), prolonged exposure (16-18 hours) to mitotic inhibitors was required for metaphase isolation; shorter exposures to mitotic inhibitors (3-6 hours) were generally sufficient for ovarian cancer cells in culture for days to weeks (long-term cultures).
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chattopadhyay1992ovariancancer
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| Authors | Chattopadhyay, S;Marinduque, B;Gilbert, F; |
| Journal | Cancer genetics and cytogenetics |
| Year | 1992 |
| DOI | DOI not found |
| URL | URL not found |
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