An infrared IgG immunoassay based on the use of a nanocomposite consisting of silica coated FeO superparticles.

A reliable, rapid and ultrasensitive immunoassay is described for determination of immunoglobulin G (IgG). It is making use of biofunctional magnetite (FeO) superparticles coated with SiO and serving as an infrared (IR) probe. The unique IR fingerprint signals originating from the transverse and longitudinal phonon modes, respectively, of the asymmetric stretching of the Si-O-Si bridges display a satisfactory resistance to optical interference from the environment. The adoption of FeO superparticles instead of FeO nanoparticles as the magnetic core warrants a controllable structure and a strong magnetic response. This facilitates the efficient purification of the probes and the alleviation of the interfacial resistance between the liquid-solid interfaces by using a magnet. The gold-coated substrate was used to immobilize goat-anti-human IgG. The analyte (human IgG) was incubated with the IR probes, and then captured by the substrate immobilized antibody with the assistance of an external magnetic field. The integral area of the IR absorption band between 1250 cm - 900 cm was chosen for quantitative assay. The limit of detection is 95 fM, which is two orders of magnitude better than that without the magnetic field. The assay time was shortened from 2 h to 1 min. High selectivity, specificity, and long-term stability of the immunoassay were achieved. The performance of the assay when analyzing blood samples confirmed the practicability of the method. Graphical abstract Schematic presentation of the infrared (IR) immunoassay based on FeO superparticle@SiO nanocomposites. The assistance of an external magnetic field reduces the incubation time and improves the detection sensitivity.