Knockdown of astrocyte elevated gene-1 inhibits proliferation and enhancing chemo-sensitivity to cisplatin or doxorubicin in neuroblastoma cells

Clicks: 342
ID: 61423
2009

Abstract

Background

Astrocyte elevated gene-1 (AEG-1) was originally characterized as a HIV-1-inducible gene in primary human fetal astrocyte. Recent studies highlight a potential role of AEG-1 in promoting tumor progression and metastasis. The aim of this study was to investigate if AEG-1 serves as a potential therapeutic target of human neuroblastoma.

Methods

We employed RNA interference to reduce AEG-1 expression in human neuroblastoma cell lines and analyzed their phenotypic changes.

Results

We found that the knockdown of AEG-1 expression in human neuroblastoma cells significantly inhibited cell proliferation and apoptosis. The specific downregulation induced cell arrest in the G0/G1 phase of cell cycle. In the present study, we also observed a significant enhancement of chemo-sensitivity to cisplatin and doxorubicin by knockdown of AEG-1.

Conclusion

Our study suggests that overexpressed AEG-1 enhance the tumorogenic properties of neuroblastoma cells. The inhibition of AEG-1 expression could be a new adjuvant therapy for neuroblastoma.

Reference Key
li2009knockdownjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Li, Xie;Chunxi, Liu;Xianrang, Song;Haiyan, Liu;Ling, Wei;Ruopeng, Sun;
Journal journal of experimental & clinical cancer research
Year 2009
DOI DOI not found
URL
http://www.jeccr.com/content/28/1/19
Keywords
chemistry Therapeutics. Pharmacology Biology (General) Medicine Pediatrics Science Diseases of the blood and blood-forming organs neoplasms. tumors. oncology. including cancer and carcinogens

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