Development of an LC-MS/MS-based method for quantitation of osimertinib in human plasma and cerebrospinal fluid.
Clicks: 207
ID: 69977
2019
The transitivity of osimertinib to cerebrospinal fluid (CSF) is of clinical concern. A quantitative LC-MS/MS method for the determination of osimertinib in human plasma and CSF was developed to evaluate its transitivity. The calibration range was 40-1000 nM in plasma and 0.8-100 nM in CSF. Accuracy and precision were within 15%. Osimertinib in the CSF but not in plasma strongly adsorbed onto the storage container. The mean adsorbed loss of osimertinib was 45.5% in CSF. Nonspecific binding in CSF was decreased by protein addition (mean loss = 5.8%). A robust validated method was developed for the quantification of osimertinib in human plasma and CSF.
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| Authors | Irie, Kei;Nanjo, Shigeki;Hata, Akito;Yamasaki, Yuta;Okada, Yutaka;Katakami, Nobuyuki;Fukushima, Shoji; |
| Journal | bioanalysis |
| Year | 2019 |
| DOI | 10.4155/bio-2018-0292 |
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