A serine/threonine-specific protein kinase of Haemonchus contortus with a role in the development.
Clicks: 226
ID: 79519
2019
In the free-living nematode Caenorhabditis elegans, the serine/threonine-specific protein kinase, AKT, is known to play a key role in dauer formation, life-span, and stress-resistance through the insulin-like signaling pathway. Although the structure and function of AKT-coding genes of C. elegans are understood, this is not the case for homologous genes in parasitic nematodes. In the present study, we explored a C. elegans akt-1 gene homolog in the parasitic nematode Haemonchus contortus, investigated its transcript isoforms (Hc-akt-1a and Hc-akt-1b), and studied expression and function using both homologous and heterologous functional genomic tools. In C. elegans, we showed that the predicted promoter of Hc-akt-1 drives substantial expression in ASJ neurons of the N2 (wild-type) strain. In H. contortus (Haecon-5 stain), RNAi (soaking) led to a significantly decreased transcript abundance for both Hc-akt-1a and Hc-akt-1b, and reduced larval development in larval stages in vitro. Chemical inhibition was also shown to block larval development. Taken together, the evidence from this study points to a key functional role for Hc-akt-1 in H. contortus.
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Authors | Di, Wenda;Gasser, Robin B;He, Li;Li, Fangfang;Liu, Xiaofang;Zhou, Caixian;Zhou, Yanqin;Fang, Rui;Zhao, Junlong;Hu, Min; |
Journal | faseb journal : official publication of the federation of american societies for experimental biology |
Year | 2019 |
DOI | 10.1096/fj.201900888RR |
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