Towards a TILLING platform for functional genomics in Piel de Sapo melons

Clicks: 188
ID: 89440
2011

Abstract

Background

The availability of genetic and genomic resources for melon has increased significantly, but functional genomics resources are still limited for this crop. TILLING is a powerful reverse genetics approach that can be utilized to generate novel mutations in candidate genes. A TILLING resource is available for cantalupensis melons, but not for inodorus melons, the other main commercial group.

Results

A new ethyl methanesulfonate-mutagenized (EMS) melon population was generated for the first time in an andromonoecious non-climacteric inodorus Piel de Sapo genetic background. Diverse mutant phenotypes in seedlings, vines and fruits were observed, some of which were of possible commercial interest. The population was first screened for mutations in three target genes involved in disease resistance and fruit quality (Cm-PDS, Cm-eIF4E and Cm-eIFI(iso)4E). The same genes were also tilled in the available monoecious and climacteric cantalupensis EMS melon population. The overall mutation density in this first Piel de Sapo TILLING platform was estimated to be 1 mutation/1.5 Mb by screening four additional genes (Cm-ACO1, Cm-NOR, Cm-DET1 and Cm-DHS). Thirty-three point mutations were found for the seven gene targets, six of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was demonstrated for a loss-of-function mutation in the Phytoene desaturase gene, which is involved in carotenoid biosynthesis.

Conclusions

The TILLING approach was successful at providing new mutations in the genetic background of Piel de Sapo in most of the analyzed genes, even in genes for which natural variation is extremely low. This new resource will facilitate reverse genetics studies in non-climacteric melons, contributing materially to future genomic and breeding studies.

Reference Key
marta2011towardsbmc Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Marta, Pujol;Christelle, Troadec;J, Monforte Antonio;Cristina, Roig;Cristina, Esteras;Meihong, Xu;Mireia, González;Fernando, Nuez;Abdelhafid, Bendahmane;Jordi, Garcia-Mas;Belén, Picó;
Journal BMC research notes
Year 2011
DOI DOI not found
URL
http://www.biomedcentral.com/1756-0500/4/289
Keywords
Biology (General) Medicine (General) Medicine Chemical technology Science (General) Science agriculture social sciences special aspects of education theory and practice of education

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