the effect of active principles of cilantro and spirulina powder on lead antagonism to zinc and iron in carassius gibelio

Our research purpose was focused towards highlighting the lead toxicity on some tissues from Carassius gibelio Bloch (Prussian carp – Cyprinidae family) after induction of chronic intoxication with lead nitrate, hydrated with ½ water molecules – Pb(NO3)2x ½H2O. By this exploratory study, we wanted to research the detoxifying potential of the active principles from lyophilized cilantro and spirulina in experimental contamination with lead, to Carassius gibelio, and their effect on lead antagonism to iron and zinc. 120 Prussian carps, weighing 22-25 g each were divided according to the following treatments for 21 days: C group (without treatment), E1 group (75 ppm Pb into water as Pb(NO3)2x ½H2O), E2 group  (75 ppm Pb into water+2% lyophilized cilantro in feed), E3 group (75 ppm Pb into water+2% lyophilized spirulina in feed). At the end of the experimental period, tissue samples (gills, muscles myotome– epaxial, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected after a starving for 12 hours, and fish euthanasia with clove oil. The analytical determination in order to establish zinc and iron concentrations, using atomic absorption spectrometer (AAS – VARIAN) was performed. Cilantro and spirulina powder additionally administered in feed, has immobilized Pb through their active principles, thus decreasing its antagonistic effect expressed to Zn, even if Zn tissue level remained low. Pb antagonistic effect shown to Fe was diminished especially in tissue sampled from individuals receiving spirulina powder in feed.